Of note, the relative band intensities of the wild-type Trp53 were decreased significantly in 4 out of 8 liver metastasized tumor samples compared with that in the parental organoid cells, while those in all spleen tumor samples were at a similar level to the control organoid, indicating that the tumor cells that carried GOF mutant Trp53 and lost wild-type Trp53 (hereafter AKTPM/LOH cells) were enriched in the metastatic tumors. This evidence concerns the gene TP53 and metastatic neoplasm.