To clarify the cellular mechanism underlying EVI5-mediated tumor progression, we established stable EVI5-knockout NSCLC cell lines (EVI5-KO cells), this was confirmed by the barely expressed level of EVI5 protein expression in EVI5-KO cells compared to that in vector transfected cells (Cas-9 cells) (Fig. 3a). This evidence concerns the gene EVI5 and neoplasm.