ATG7 and malignant colon neoplasm: To assess the crosstalk between autophagy and apoptosis in 6-shogaol and FU-treated colon cancer cells, three autophagy (LCI/II, Beclin-1, Atg-7) and three apoptosis-related proteins (Bcl-2, Bax, Caspase-3) were selected for the immunocytochemical evaluation (Fig. 3).