Using separate NME1 and 1/3-pHis immunoprecipitation experiments with lysates from SK-N-BE(2) and SK-N-AS neuroblastoma cell lines and phosphopeptide immunoaffinity purification using a non-acidic approach [34] with tryptic digests of proteins extracted from orthotopic xenograft tumors (Supplementary Data 3), we were able to identify by mass spectrometry a number of candidate NME1 histidine kinase target proteins, based on their association with NME1and their pull down with anti-pHis antibodies (Figure 7A). This evidence concerns the gene NME1 and neuroblastoma.