In all (n = 105) or cirrhotic patients (n = 93) with HCC, lower AST, ALT, total bilirubin, AFP, FIB-4, and platelets 12M; and higher AST 12M and FIB-4 12M were significantly associated with the subgroup with △FIB-4 ≥ 0 (Tables S10 and S11). This evidence concerns the gene AFP and hepatocellular carcinoma.