Autophagy activation was confirmed by Western blot when researchers observed increases in MAP1LC3-II and decreases in sequestosome 1/p62 (p62) expression in placentas from patients with hypertensive disorder [23], and the presence of autophagic vacuoles in the syncytial layer of preeclamptic placentas [26]. The gene discussed is SQSTM1; the disease is hypertensive disorder.