ROC curve analysis showed that the CD25‐MUC1‐VEGFR1 combination‐based ELISA showed the largest AUC of 0.883 (95% CI 0.844–0.923) with a sensitivity of 49.6% against the specificity of 95.0% in patients with early‐stage NSCLC. Here, MUC1 is linked to non-small cell lung carcinoma.