The in‐house ELISA made with the CD25‐MUC1‐VEGFR1 combination showed significantly lower IgG levels in NSCLC patients than control subjects (Z = −12.978, P < 0.001), and both male and female patients contributed to the decrease in plasma IgG levels, while plasma anti‐POU5F1 IgG levels were significantly lower in female patients with NSCLC than female control subjects (Z = −2.477, P = 0.013) (Table 4). The gene discussed is POU5F1; the disease is non-small cell lung carcinoma.