CCR2 and neoplasm: In order to verify CCR2 expression on neutrophils in the brains of tumor-bearing animals, we performed flow cytometry for CCR2 (using an anti-CCR2 antibody) on Ly6G+ circulating, liver-infiltrating, and brain-infiltrating neutrophils in both sham and PDAC-bearing animals at 10 d.p.i. As expected, we observed minimal CCR2 expression on circulating neutrophils in sham animals.