To evaluate the function of Ena/VASP proteins in cell migration in a comparably fast mesenchymal cell type, we sequentially inactivated the three Ena/VASP paralogues Evl, VASP and Mena using CRISPR/Cas9 technology in B16-F1 mouse melanoma cells (Supplementary file 1). The gene discussed is ENAH; the disease is melanoma.