More importantly, a recent large scale genomic study of more than 5,000 tumor samples definitely showed that a low-allele-fraction mutations in cancer samples is a surprisingly frequent condition, with a routine detection of hotspot mutations in actionable genes such as EGFR, KRAS, PIK3CA and BRAF with an allele fraction below 10% in about 20% of clinical samples (23). This evidence concerns the gene PIK3CA and neoplasm.