These samples were analyzed by means of a custom NGS amplicon approach targeting key genes frequently altered in GIST (KIT, PDGFRA, BRAF, NRAS, KRAS, SDHA, SDHB, SDHC, SDHD, and NF1), reaching an average depth of coverage of 295X. The gene discussed is SDHC; the disease is gastrointestinal stromal tumor.