For instance, in osteosarcoma cells, the EMT-TFs, such as Twist, Snails, and Zebs are over-activated (19) and the genes such as TIM3 (20), ST6GAL1 (21), TRIM66 (22), UHRF1 (23), and CYR61 (24) contribute to the positive regulation of EMT-TFs. This evidence concerns the gene TRIM66 and osteosarcoma.