We found that only incubating VMECs with CX3CL1 increased the TEM of tumor cells but that pre-inhibition of Src/P115-RhoGEF/ROCK signaling in the VMECs significantly reversed CX3CL1-induced VMECs barrier hyperpermeability and the high rate of tumor cell TEM (Figures 13A,C). Here, CX3CL1 is linked to neoplasm.