Using RNA interference, we generated LN18 and U87 glioma cells stably depleted of CSF2 and for each glioma cell line we selected two clones (shCsf2) with the highest reduction of CSF2 mRNA and CSF2 protein levels, as determined by qPCR (Fig. 2a, c) and ELISA (Fig. 2b, d), respectively. This evidence concerns the gene CSF2 and central nervous system cancer.