The human cell line activation test (h-CLAT), the U937 cell line activation test (U-SENSTM), and the interleukin-8 reporter gene assay (IL-8) (OECD, 2018b) are also validated in vitro methods that evaluate activation of dendritic cells – the third key event in the AOP for skin sensitization. Here, CXCL8 is linked to neoplasm.