Compared to their controls, the percentage of AKT phosphorylation, measured by the ratio between phosphorylated AKT (pAKT) and total AKT, was greatly increased in the primary CRC lines, HCT116 BMAL1-KD (p=0.0207) and SW480 BMAL1-KD (p=0.0302), and slightly increased in the metastatic SW620 BMAL1-KD (p=0.0141) cells (Figure 2A). The gene discussed is BMAL1; the disease is colorectal carcinoma.