TNF and myelofibrosis: Gene set enrichment analysis (GSEA) of all expressed genes pre-ranked according to their differential expression between myelofibrosis and control MkP showed significant enrichment of metabolic (e.g., oxidative phosphorylation and fatty acid metabolism) and inflammatory pathways (e.g., transforming growth factor α [TNF-α] signaling and interferon gamma response) in myelofibrosis MkPs (Figure 5B).