Serum concentrations of T and LH were significantly higher in all the PCOS groups (include PCOS group and PCOS+Gln groups) than the control group (Fig. 2a, c), and the concentrations of T were increasing with the dosage of supplementation of glutamine, but the concentrations of LH were decreasing with the dosage of supplementation of glutamine. The gene discussed is PLOD1; the disease is polycystic ovary syndrome.