The heterogeneity of enteroendocrine cells was further explored by Yan and colleagues (2017) using scRNA-seq.49 Among the intestinal stem cells, Bmi1+ cells were found to be the most enriched for enteroendocrine markers, including transcription factor Prox1, a transcription factor previously associated with the progression of colonic cancer.44, 50 Prox1+ intestinal epithelial cells were isolated and used for scRNA-seq using a droplet-based system. Here, PROX1 is linked to malignant colon neoplasm.