Specifically, the mammary gland tissues and spontaneous tumors were harvested from mice 6 h after HS201 (100 nmol/mouse) injection, enzymatically digested into single breast tumor cells or mammary epithelial cells, stained with CD24 and CD45, and analyzed by an LSRII flow cytometer (Fig. 4d). This evidence concerns the gene PTPRC and breast neoplasm.