Furthermore, when inflammasome activation was inhibited by MCC950 or extracellular KCl during infection, the number of ALG-2 events per cell remained constant while ASC-speck formation was inhibited, confirming that PM damage happens prior to NLRP3 inflammasome activation (and is not caused e.g. by active GSDMD) (Fig. 7e; Supplementary Fig. 7b). This evidence concerns the gene GSDMD and infection.