SCN9A and hereditary sensory and autonomic neuropathy: To discover peptide activators that can induce pain and recover pain responses in Nav1.7-related CIP, we first fractionated 15 crude venoms (from ten spiders and five snakes) using semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC) and collected a total of 110 fractions (5–10 fractions per venom).