Cancer cells stably expressing GFP were visualized in all four conditions to analyze the number of migrating cancer cells and the migration distance from the edge of the lumen using Image J. Qualitatively, an increase in the number of migrating cells was observed in a fibronectin-rich matrix as compared to the collagen only matrix, regardless of the type of fibroblasts present (Figure 2b,c). Here, FN1 is linked to cancer.