Recently, many biomarkers have been widely used for HCC diagnosis, such as alpha-fetoprotein (AFP) [4], suppressor with morphogenetic effect on genitalia-1 (SMG-1) [5, 6], Golgi membrane protein 1 (GOLM1) [7], BarH-like Homeobox 2 (Barx2) [8], des-γ-carboxy prothrombin (DCP) [9], glypican-3 [10], cytokeratin-19 [11], Golgi protein-73 [12], osteopontin [13], miR-122, and PIVKA-II [14]. This evidence concerns the gene AFP and hepatocellular carcinoma.