To explore the potential contribution of antioxidant system in CRIF1-increased ROS level in HCC, the effect of CRIF1 on the activities of three major antioxidant enzymes17,18, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were determined. This evidence concerns the gene GADD45GIP1 and hepatocellular carcinoma.