As showed in Fig. 4a, according to the heat map of protein expression of 278 RCC samples in TCGA database, AKT, beta-Catenin, GSK3-alpha-beta, PTEN, RAD50, BRD4 protein expression level was elevated significantly in all tested proteins, so we considered that those molecules played vital roles in RCC. This evidence concerns the gene AKT1 and renal cell carcinoma.