As showed in Fig. 4a, according to the heat map of protein expression of 278 RCC samples in TCGA database, AKT, beta-Catenin, GSK3-alpha-beta, PTEN, RAD50, BRD4 protein expression level was elevated significantly in all tested proteins, so we considered that those molecules played vital roles in RCC. The gene discussed is BRD4; the disease is renal cell carcinoma.