In this study, we demonstrated that hucMSC-Ex could increase CK1δ and β-TRCP expression and promote YAP ubiquitination and degradation in the kidneys of UUO rat models, which inhibited ECM deposition and alleviated renal fibrosis, indicating that hucMSC-Ex may represent a new strategy for the treatment of renal fibrosis. Here, BTRC is linked to renal fibrosis.