Firstly, we overexpressed NKILA or NC in U87 and A172 cells with lentivirus (Lv-O.E.-NC or Lv-O.E.-NKILA), 48 h later, we determined the intracellular and extracellular (supernatant of glioma cells) NKILA levels in U87 and A172 cells with different transfection by qRT-PCR. This evidence concerns the gene NKILA and central nervous system cancer.