Moreover, Western blot analysis showed that Vitamin K2 stepwise elevated the expression of some glycolytic proteins or enzymes, such as GLUT-1, Hexokinase II (HK2), PFKFB2, LDHA and PDHK1, in bladder cancer T24 (Fig. 1E) and EJ cells (Fig. 1F). This evidence concerns the gene PFKFB2 and urinary bladder cancer.