TARDBP and amyotrophic lateral sclerosis: No cytoplasmic TDP43 accumulations were observed in normal 8-month-old mice administered ALS-CSF i.c.v. for 14 days (Supplementary Fig. S1 a, b) even though we did observe motor phenotypes in response to ALS-CSF with normal mice albeit the magnitude was less pronounced than with hTDP43 transgenic mice.