Immunofluorescence microscopy of cortical and hippocampal sections revealed cytoplasmic TDP43 mislocalization in response to ALS-CSF (Fig. 5e) which was confirmed with a quantitative analysis demonstrating a decreased nuclear to cytoplasmic (N/C) TDP43 intensity ratio in the cortical sections from ALS group (Fig. 5 h). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.