After the transfection efficiency was verified by western blot (Fig. S5a), CCK-8 and colony formation assays showed that the cell viability was inhibited after CXCL12 knockdown, and wound healing, transwell invasion assays revealed that CXLC12 knockdown decreased cell migration, invasion of melanoma cells (Fig. 5d-g). This evidence concerns the gene CXCL12 and melanoma.