We demonstrate that hsa-miR-20b-5p and hsa-miR-363-3p affect the expression of two important tumor suppressor genes, PTEN and BIM. We also show that mimicry and inhibition of these miRNAs have impact on the survival of T-ALL cells in vitro, by affecting spontaneous apoptosis and proliferation. Here, PTEN is linked to acute lymphoblastic leukemia.