Figure 1B has the identical loading plan as in Figure 1A, but the blot was incubated with anti-Aβ antibodies (6E10) which bound to the APP protein. Here, APP, APP-His protein bound to beads or Tau bound to APP-His beads demonstrated 110 kD band (lanes 1, 2, 5, 6, 9, 10) which is the MW of APP. Thus, Figure 1A,B confirm that the two proteins which are the main components contributing to AD bind to each other. Here, APP is linked to Alzheimer disease.