Indeed, here we present two GD cellular models obtained by editing GBA1. After developing an editing workflow using Human Embryonic Kidney cells 293 (HEK), we edited a human monocytic cell line deriving from an acute monocytic leukemia patient (THP-1) and the glioblastoma U87 cell line (U87) to be used as a model of GD monocytes and glial cells, respectively. Here, GBA1 is linked to acute monocytic leukemia.