After injection of B7-H3-ICG, glands with histologically confirmed DCIS showed a statistically significantly higher average sPA molecular B7-H3 signal (22.7 ± 40.2 a.u.; P < 0.05) compared with normal murine mammary glands which showed an average of 3.2 ± 2.0 a.u molecular imaging signal. The gene discussed is CD276; the disease is ductal breast carcinoma in situ.