In addition, we also used co-expression and GSEA methods to analyse the pathways that CDCA8 may affect in bladder cancer, and found that CDCA8 may participate in important pathways such as Cell cycle and P53 signalling pathway; We also found that the overexpression of CDCA8 was significantly related to the mutations of RB1 and P53. The gene discussed is CDCA8; the disease is urinary bladder cancer.