To evaluate whether pharmacologic suppression of shh signaling in pancreatic cancer cells could reverse the effect of CM from Cav-1-knockdown PSCs on Aspc-1 proliferation, a [3H]thymidine incorporation assay was performed with pancreatic cancer cells cultured with CM from Cav-1-knockdown PSCs for 48 h with or without cyclopamine, a specific inhibitor of shh signaling. The gene discussed is CAV1; the disease is pancreatic neoplasm.