To evaluate whether pharmacologic suppression of shh signaling in pancreatic cancer cells could reverse the effect of CM from Cav-1-knockdown PSCs on Aspc-1 proliferation, a [3H]thymidine incorporation assay was performed with pancreatic cancer cells cultured with CM from Cav-1-knockdown PSCs for 48 h with or without cyclopamine, a specific inhibitor of shh signaling. This evidence concerns the gene CAV1 and familial pancreatic carcinoma.