In line with the upregulation in SIRT3, an increment in phospho-AMPKα was found in group B. But we did not find a significant difference neither in total AMPKα and phospho-AMPKα/total AMPKα ratio nor in PGC-1α expression among groups, yet an increment tendency was observed in patients with metabolic diseases. The gene discussed is PPARGC1A; the disease is metabolic disease.