Moreover, knockdown of AFAP1-AS1 expression by pSilence-AFAP1-AS1 transfection reduced the viability and colony formation capacity of MDA-MB-231 cells (Fig. 2C,D), whereas miR-145 co-transfection rescued tumor cell viability and colony formation ability (Fig. 2C,D). The gene discussed is AFAP1; the disease is neoplasm.