In this study, we first analyzed AFAP1-AS1 expression in TNBC and found that AFAP1-AS1 expression was significantly higher in TNBC than in other subtypes of breast cancer using TCGA dataset (Figure S1) We also found that expression levels of miR-145 and MTH1 in TNBC were obviously lower and higher than those in luminal breast cancer, respectively (Figures S2 and S3). This evidence concerns the gene AFAP1 and breast cancer.