The ability of the cancer cells to overcome the anoikis is acquired by EMT-mediated mesenchymal cell phenotype, which increases level of the anti-apoptotic gene, Bcl-2 and activation of the pro-survival signaling including AKT and ERK signal pathways through EMT-related factors including Snail, ZEB1/2, Twist, NF-κB, and HIF1/2. The gene discussed is TWIST1; the disease is cancer.