PINK1 depletion in mouse embryonic fibroblasts from PINK1 knockout mice also impaired lysosomal activity and led to enlargement of lysosomal vacuoles [73] and silencing of DJ-1 in M17 neuroblastoma cells led to an accumulation of autophagy markers, in addition to mitochondrial membrane depolarization and mitochondrial fragmentation [341]. This evidence concerns the gene PINK1 and neuroblastoma.