As presented in Figure 7A, real-time PCR analysis showed that TNFα mRNA significantly increased in the NASH group compared with the control group (3.14±0.1 vs 1, P<0.01) while in the NASH+allantoin group it significantly decreased compared with the NASH group (1.57±0.11 vs 3.14±0.1, P<0.01). This evidence concerns the gene TNF and metabolic dysfunction-associated steatohepatitis.