PTT with anti-DLL4-conjugated TNPs inhibits tumor growth in SSIL2Rγ- (DLL4-high) as SS.BN3IL2Rγ- (DLL4-low). To assess the impact of anti-DLL4-conjugated TNPs on SSIL2Rγ- and SS.BN3IL2Rγ- rats were implanted with MDA-MB-231(231LUC+) triple negative breast cancer cells in the inguinal mammary fat pad. The gene discussed is DLL4; the disease is triple-negative breast carcinoma.