We assessed mRNA expression in individual cells isolated from the four lung cancer cell lines, H2228, H460, HCC78, and PC9 (Figure S4), and from the blood samples withdrawn from three patients with NSCLC, LP25, LP38, and LP39, before starting their EGFR-TKIs therapy, using WBCs as a control (Figure S5). The gene discussed is EGFR; the disease is lung carcinoma.