AGT and endothelial dysfunction: In order to examine whether NADPH oxidase–driven ROS production is involved in endothelial dysfunction induced by AngII, we measured the relaxation to ACh in aorta (Supplementary Figure S3) and the phosphorylation of VASP at Ser239 by immunofluorescence in RAECs incubated with the specific pan-NOX inhibitor VAS2870.