In vivo, we found that NE-THCQ attenuated renal fibrosis after UUO surgery, and this conclusion was supported by less pathological alterations in Masson’ s stain, lower expression of α-SMA, Col-I and Col-III and higher expression of E-cadherin in the NE-THCQ group compared to the UUO group. The gene discussed is CDH1; the disease is renal fibrosis.