1. Significant decrease of total inflammatory cell count, eosinophils, neutrophils, lymphocytes, and macrophages: ≥ 2.5 mg/kg2. Significant decrease of IL-4, IL-5, and IL-13 in BALF: ≥ 2.5 mg/kg3. Significant decrease in IgE production: ≥ 2.5 mg/kg4. Significant alleviation of OVA-induced airway hyperresponsiveness (expressed at enhanced minute pause (Penh) that reflects pulmonary resistance when treated with 10 mg/kg methchacholine: ≥ 2.5 mg/kg5. Significant decrease in p-p65/β-actin ratio and p-IκB/β-actin ratio: ≥ 2.5 mg/kg. The gene discussed is IL4; the disease is airway hyperresponsiveness.