A recent study also revealed that downregulation of MCU suppressed cell motility and reduced tumor growth in triple-negative breast cancer by regulating store-operated Ca2+ entry.30 Moreover, a study from our group revealed that MCU promotes the metastasis of HCC cells via the nicotinamide adenine dinucleotide + /sirtuin 3/superoxide dismutase 2 signaling pathway.11 To the best of our knowledge, the present study is the first to provide evidence that MCU-mediated mitochondrial Ca2+ uptake is crucial for CRC cell growth by increasing mitochondrial biogenesis and ROS/NF-κB signaling. This evidence concerns the gene SIRT3 and neoplasm.