For example, while TP53, PMS2, and RET are already reported as genes with germline alterations in individuals with Ewing sarcoma, we identified germline P/LP variants in ATM (1 patient), BRCA1 (1), CHEK2 (3), GJB2 (1), LZTR1 (1), and POLE (1) genes in cases with Ewing sarcoma (Supplementary Fig. 6). This evidence concerns the gene GJB2 and Ewing sarcoma.