POLE and Ewing sarcoma: For example, while TP53, PMS2, and RET are already reported as genes with germline alterations in individuals with Ewing sarcoma, we identified germline P/LP variants in ATM (1 patient), BRCA1 (1), CHEK2 (3), GJB2 (1), LZTR1 (1), and POLE (1) genes in cases with Ewing sarcoma (Supplementary Fig. 6).