BRCA2 and osteosarcoma: We found three pathogenic germline variants (Methods), one nonsense mutation in TP53, and two frameshift indels in BRCA2 and GJB2 genes in two patients with osteosarcoma, which were further confirmed by Sanger sequencing (Table 1; Fig. 1a, b; Supplementary Data 3; Supplementary Fig. 2).