To gain insights into the effect of GLYR1 downregulation on apoptosis in CRC cells, we first performed CCK8 assays and calculated the half maximal inhibitory concentration (IC50) of 5-FU in SW480 (8.185 ± 0.1935 μg/ml) and SW620 (5.293 ± 0.1575 μg/ml) cells (data not shown). This evidence concerns the gene GLYR1 and colorectal carcinoma.