TNF and breast cancer: Mock vaccinated mice had a predominantly Th1 CD4+ T-cell response with production of IFN- γ and CD8+ T-cells that were positive for IFN-γ or granzyme B. In contrast, BC-FAST vaccinated mice, both with and without ICI treatment, had T-cells that could produce effector cytokines (IFN-γ, TNF-α or IL-2) and degranulate (Granzyme B).